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Monday, 26 August, 2019
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Birefringence analysis is the study of the anisotropic interaction of matter with light.

Birefringence is resulting from the so-called intrinsic birefrigence and from the so-called form birefringence. Intrinsic birefringence is mainly resulting from the anisotropic properties of the molecules itself, whereas form birefringence is resulting from anisotropic polarisabilities resulting if elongated structures are present in a media with a different refraction index.

Taorad uses these anisotropies for both studying intrinsic birefringence, as observed in crystals, as well as form birefringence, as observed in tissues like bone, cartilage,  brains white and even gray matter.

In order to detect those birefringencies, light with well defined properties (e.g. polarization states) is used to probe the sampe and the resulting properties of the light are analyzed in order to deduce the birefgringence of the subject under study.

The following images shoes its application in the area of protein crystallization and tissue imaging (cartilage, bone and brain). With Taorads automated microscope LMP-1, even large samples as whole human brain slices can be analyzed at resolutions better than 1 micrometer. For a single human brain slice, nearly 10.000 images are automatically taken and more than 6,5 billion fourrier transforms calculated.

All samples shown are unstained and the colors are representing one of the properties of the birefringence!


different anisotropic properties of protein crystals, cartilage, bone and brain (twice)